Products

DAISOGEL is the world-leading bulk silica chromatography media brand of OSAKA SODA Co., Ltd., used principally for liquid chromatographic analysis and for the separation and purification of active pharmaceutical ingredients and high-value compounds. Production takes place at the Amagasaki plant in Osaka, Japan, under the direction of a specialist technical team.

The plant operates a rigorous quality management system; production conforms to ISO 9001, and documentation relating to US FDA-DMF certification can be supplied for chemically bonded products. These manufacturing standards give DAISOGEL products high quality and high purity, meeting or exceeding the audit requirements of pharmaceutical manufacturers worldwide.

Since the launch of DAISOGEL in 1992, the brand has supplied silica chromatography products of high purity, high stability and sustained availability. Offices or agencies are maintained in China, Japan, the United States and Europe, backed by more than 40 years of experience in chromatographic analysis, separation and purification, and manufacturing.

DAISO controls raw materials and product quality at every production stage, releasing only material that meets manufacturing specifications. Pore size, specific surface area, pore volume and pore size distribution are determined by BET and porosimetry; particle size and its distribution by sedimentation and electrical sensing zone methods. All bonded-phase media additionally undergo pH, volatile matter and other selectivity testing, with the resulting data supplied to customers. Metal impurities are determined by XRF and ICP, and surface condition is examined by optical and electron microscopy.

A DAISOGEL model designation has five segments, for example SP-120-10-ODS-RPS：

The ODS-PK series is our highest-grade C18 chromatography media, developed on a holistic design concept. It offers excellent alkali resistance, higher mechanical strength and excellent loading capacity, and is used for the separation, purification and scale production of synthetic peptides, recombinant peptides/insulin, fish oil/EPA, EPO, cannabinoids and small molecules. Physicochemical performance, versatility and stability are all strong.

In dry spherical silica compression testing, the PK series showed 15% less particle fracture than competing spherical silica products.

The ODS-P series is a C18 medium built on an ultra-high-purity spherical porous silica matrix, with very high specific surface area and loading capacity. It separates both hydrophilic and hydrophobic compounds well and is highly versatile for small molecules. Its good hydrophilicity allows use even with a 100% aqueous mobile phase, making it well suited to synthetic peptides and other small molecules.

"P" denotes the "ultra-high purity" silica grade, with Al, Fe, Ti and Zr metal impurities below 10 ppm.

The ODS-HP series reversed-phase C18 media uses advanced proprietary bonding and endcapping technology, giving a very high carbon load of 24% and a wide pH tolerance. Its very high specific surface area of 450 m²/g extends compound retention while providing higher loading, making it suited to small molecules and peptides with demanding separation requirements, and offering better selectivity for particularly difficult separations.

HP stands for "High Performance"; this series is intended for compounds that are especially difficult to separate.

The RPS-ODS series is the most classic and most popular reversed-phase C18 medium. Its good acid resistance makes it an ideal choice for small-molecule separations, including small peptides, oligonucleotides, small-molecule natural products and cannabinoids.

For efficient separations, the 200 Å pore stationary phase strikes a good balance between surface area, separation speed and solvent consumption.

The ODS-BP series reversed-phase C18 media has distinctive hydrophilicity, can be used with a 100% aqueous mobile phase, and has a relatively low carbon load of 10%–15%, giving better selectivity for hydrophilic or polar compounds. It is applied to small peptides, nucleotides, organic acids and small sugars.

The ODS-BP series shows high baseline stability and sensitivity even at neutral pH without buffer salts or ion-pairing reagents, making it particularly suitable for LC-MS.

The distinctive surface bonding technology avoids the incomplete extension of long C18 alkyl chains in low-alcohol solutions, so the phase remains well extended even in purely aqueous systems. Typical applications are the separation of biological macromolecules and metabolites such as oligosaccharides, amino acids, small peptides, nucleotides and organic acids.

The ODS-BIO series C18 media offers strong acid and alkali resistance across a wide pH range, making it particularly suitable for the separation of peptides, small proteins, insulin and oligonucleotides.

The ODS-BIO series is the first choice for separating medium to somewhat larger molecules and unknown compounds; where lower sample carryover is required, the C8-BIO and C4-BIO series may be used instead.

Improved high-density bonding and full endcapping make it best suited to separating or purifying lower molecular weight compounds, particularly small peptides. Substantial improvements in durability and acid/alkali resistance allow prolonged use under acidic mobile phase conditions, with regeneration by flushing with aqueous NaOH.

The C8-PK series is an advanced octyl bonded reversed-phase silica medium developed on a holistic design concept using proprietary bonding and endcapping technology. It offers strong acid and alkali resistance together with high mechanical strength and loading capacity, and is applied to the preparative purification of recombinant and synthetic peptides such as insulin, semaglutide and tirzepatide.

For insulin separation and purification, the C8-PK series gave a recovery 22.5% higher than competing brands.

The C8-HP series is an octyl bonded silica medium with the highest carbon load in the range, developed using advanced bonding technology. Its carbon load reaches 15.5%, and it can be used across a wide pH window with strong acid and alkali resistance.

The C8-HP series is recommended for purification of more hydrophobic samples.

The C8-BIO series offers good acid and alkali resistance across a wide pH range, making it well suited to preparative purification of peptides, recombinant proteins (insulin) and oligonucleotides.

For purification of medium to larger molecules, the C8-BIO series achieves better resolution and recovery.

DAISOGEL C8-P is fully endcapped octyl bonded silica, well suited to compounds strongly retained on C18. Four pore sizes are available — 6, 12, 20 and 30 nm — allowing the separation to be optimised to solute molecular size. The 20 and 30 nm grades are widely used for highly hydrophobic macromolecules.

The C4Ph-HP series is a novel alkyl-phenyl bonded spherical silica chromatography medium with distinctive compound recognition. Its separation principle resembles that of conventional C18 and C8 media, providing hydrophobic interaction, while additionally offering π-π interaction recognition that those phases lack.

The C4Ph-HP phenyl series combines a well-judged functional group type with an appropriate carbon load density. For purification of aromatic compounds it achieves better resolution and higher loading capacity than conventional phenyl bonded phases.

The C4-PK series is our highest-grade C4 chromatography medium, developed on a holistic design concept. It offers excellent alkali resistance, higher mechanical strength and excellent loading capacity, and is applied to the separation and purification of medium to larger recombinant proteins. Physicochemical performance, versatility and stability are all strong.

PK series bare silica withstands flushing with more than 170 column volumes of dilute NaOH solution before particle dissolution — 50% more CV than competing products.

The BIO series C4 bonded phase is suited to purification of small peptides, microbial proteins (insulin) and oligonucleotides, with a wide applicable pH range.

BIO series C4 media is stable between pH 1.5 and 11; the wide-pore 300 Å BIO series C4 grade is well suited to large proteins.

Where a particular application calls for distinctive selectivity, this non-endcapped C4 medium can separate such samples through silanol interactions.

For the NP series C4, combining silanol hydrogen bonding with C4 hydrophobicity is only a beginning; further "unexplored territory" in non-endcapped bonded phases is to come.

The C4-P series uses a butyl bonded phase with moderate hydrophobicity; SP-300-20-C4-P is well suited to purification of erythropoietin (EPO).

"P" denotes ultra-high-purity silica, with Al, Fe, Ti and Zr metal impurities below 10 ppm.

The 12, 20 and 30 nm pore grades are suited to separation of peptides and proteins. Wide-pore silica in particular has a smaller surface area, suiting it to the separation of denatured proteins and of compounds spanning a wide hydrophobicity range.

The P series APS is an aminopropyl bonded silica medium, suited to carbohydrate purification and to hydrophilic interaction liquid chromatography (HILIC).

As regards mobile phase, it can be used under both normal-phase and reversed-phase conditions, particularly for separating carbohydrates in acetonitrile/water. Basic substances that unmodified silica cannot separate may also be attempted on this medium under normal-phase conditions. Simple elution systems such as hexane/ethyl acetate or chloroform/methanol can be used without polar additives.

Daiso APS uses aminopropylsilane as the bonded phase on an ultra-high-purity silica base. The 60 Å small-pore grade is suited to normal-phase and HILIC separation of small polar molecules.

The Daiso SP-P (spherical ultrapure) series is a spherical, fully porous silica with total metal impurities (aluminium, iron, titanium and zirconium) below 10 ppm. It is manufactured under tightly controlled conditions, giving a uniform particle distribution and good lot-to-lot reproducibility of particle distribution, pore size and surface area, while avoiding the small pores that degrade performance.

Analytical grades are available at 3 μm, 4 μm and 5 μm; preparative grades at 7 μm, 10 μm, 15 μm and 20 μm. High-purity 40–60 μm spherical silica is an economical option for medium- and low-pressure chromatographic separation.

The SP-100-P series is the newest member of the DAISOGEL bare silica range, available in four particle sizes: 3 μm, 5 μm, 10 μm and 15 μm. Its 10 nm pore size gives the medium a very high surface area — under 600 mg of the material has a surface area considerably greater than an entire doubles tennis court.

The high surface area SP-100-P series has mechanical strength suited to DAC (dynamic axial compression) columns.

The Daiso SWP (super wide pore) series has a uniform pore and particle size distribution, low metal content and enhanced mechanical stability. Its wide pores suit it well to the separation of large molecules. Pore sizes of 100 nm and 200 nm are available, for separation of proteins, oligonucleotides and biomolecules that cannot be separated on the narrow-pore silica generally available. The SWP series includes both bare silica and a range of chemically bonded phases.

Daiso's 8 μm media is the latest high-pressure silica introduced, available with a range of bonded phases. It offers better resolution and better pressure tolerance, suiting it to dynamic axial compression (DAC).

COMPASS was created to address the difficulty of choosing among media types. It is not a conventional screening kit but a pre-orientation set of four columns with entirely different separation mechanisms, helping you decide quickly on the most suitable bonded phase direction before large-scale screening begins, and cutting time and cost substantially. It is particularly useful for development projects with no historical reference molecule.

The COSMOSIL bulk media series from Nacalai Tesque (Japan) covers C18, C4, phenyl, HILIC and other bonded phases, and is widely recognised in academic and pharmaceutical work worldwide. Microwants is the exclusive distributor for mainland China, holding mainstream specifications in stock and supplying an official lot certificate with each batch.

General-purpose silica-based chromatography media for preparative separation and purification. Porous, non-porous and core-shell types are available, with pore size, particle size and bonded phase customisable to requirement, covering gram-scale laboratory purification through kilogram-scale production.

Years of technical accumulation have given the company a complete industrialization platform spanning laboratory R&D, pilot scale-up and full production, with mature process development, large-scale manufacturing capability and a stable production record — carrying modified silica, specialty bonded phases and polymer media efficiently from development through to industrial manufacture.

General-purpose silica-based chromatography media for preparative separation and purification. Porous, non-porous and core-shell types are available, with pore size, particle size and bonded phase customisable to requirement, covering gram-scale laboratory purification through kilogram-scale production.

Ion exchange media formed by cross-linking different ligands onto the surface of cellulose microspheres, offering good flow properties together with mechanical and chemical stability. They are suited to separation and purification of proteins, peptides and other biomolecules at every chromatographic step from laboratory scale to production scale-up. Applications include antibody therapeutics, growth factors, albumin, enzymes, nucleic acids, vaccines and blood products.

Common features: spherical bridged cellulose particle matrix; operating pressure > 0.2 MPa, linear velocity up to 1200 cm/h, back pressure < 0.3 MPa; tolerates in-line cleaning with 0.5 M NaOH. BSA/lysozyme were the proteins used to determine dynamic binding capacity.

The MAX series uses new bridging technology on the cellulose base bead to graft ion-exchange ligands onto a dextran polymer layer, allowing the target protein to diffuse rapidly into the pores and giving excellent dynamic binding capacity (DBC). All MAX IEX grades maintain a stable dynamic capacity irrespective of flow rate, with linear velocity up to 500 cm/h.

Operating pressure is < 0.3 MPa throughout. MAX Q-hv and MAX Butyl HS are designed specifically for purification of polysaccharide vaccines such as pneumococcal capsular polysaccharide.

A strong cation exchange medium designed to remove aggregates from the monoclonal antibody eluate following Protein A affinity capture. A polymer bearing ion-exchange ligands is grafted onto the particle surface, and its distinctive molecular structure discriminates selectively between antibody monomer and aggregate, achieving aggregate removal.

An alkali-tolerant Protein A affinity medium for monoclonal antibody purification. It offers good flow characteristics, low ligand leakage and high dynamic binding capacity, tolerates in-line cleaning (CIP) and remains stable on repeated use, enabling rapid and efficient downstream mAb purification.

Sulfate is an affinity medium with a heparin-mimetic ligand, commonly used to separate and purify vaccine viruses (influenza, rabies, Japanese encephalitis and others). Virus is typically adsorbed under low-salt conditions and eluted under high salt, and virus culture is not restricted to egg- or cell-based methods. The MAX DexS series uses dextran sulfate — similar to animal-derived heparin — as the ligand on the cellulose base bead; DexS-HbP suits heparin-binding protein purification and DexS-VirS suits purification of viruses and virus-like particles (VLPs), the two differing in the length of the dextran sulfate polymer.

A chromatography medium for the separation and purification of DNA-binding proteins. Because the phosphate ligand resembles the structure of DNA, it has affinity for DNA-binding proteins; its negative charge also gives it cation exchange functionality, so it can be used as a cation exchange medium. In mRNA in vitro transcription (IVT) processes it can be used to purify T7 RNA polymerase and other nucleic-acid-related enzymes, with enzyme activity recovery in the eluted fractions reaching around 70%.

Note: for the 50 mL / 500 mL pack sizes, the English catalogue (N3) lists 19545 / 19546 while the Chinese catalogue (N1_V33) lists 19525 / 19526; please order against the current official catalogue. A high-capacity grade Cellufine Phosphate HC(for mRNA polymerase purification) is also available on request.

An activated support medium for immobilising antibodies, antigens and other ligands. Built on rigid spherical cellulose microspheres with wide pores and high ligand density, the cellulose backbone gives very low non-specific adsorption and avoids the ligand leakage often seen with agarose. The aldehyde functional group reacts with primary amines on the ligand to form a Schiff base, which is converted by mild reductive amination into a highly stable covalent linkage.

A chromatography medium with poly(ε-lysine) as the ligand on a cellulose base bead, binding endotoxin present in the sample selectively and removing it. The poly-lysine ligand is a 25–35 residue lysine polymer produced by the microorganism Streptomyces albulus. ET Clean L has a large exclusion limit, suiting larger proteins; ET Clean S has a smaller exclusion limit, suiting smaller molecules.

A second-generation high-flow Cellufine medium based on highly cross-linked base bead technology, offering a range of hydrophobicities for fine adjustment of antibody HIC separation. Phenyl EX belongs to the HIC series and can be used in flow-through mode for two-step antibody purification, removing mAb aggregates at low conductivity (around 6 mS/cm) while reducing equipment corrosion and the risk of buffer precipitation. MAX Phenyl (standard), MAX Phenyl LS (low ligand) and MAX Butyl offer different combinations of resolution and capacity, with resolution in the order MAX Phenyl > MAX Phenyl LS > MAX Butyl; MAX Butyl HS is a high salt-tolerance grade for polysaccharide vaccine purification.

Polyclonal IgG 10% DBC: MAX Butyl 17, MAX Phenyl 19, MAX Phenyl LS 30 mg/mL. After CIP with 0.5 M NaOH plus 30% isopropanol, Phenyl EX showed essentially unchanged binding performance over 60 cycles.

A mixed-mode medium for further polishing of monoclonal antibodies after Protein A separation in antibody manufacture. Modified with primary amine (polyallylamine) and butyl groups, it binds the target protein under high-salt, salt-tolerant conditions while removing HCP and other impurities.

GCL-2000HF offers a very wide separation range (MW 50 – 3000 kDa), suited to the separation and purification of large proteins of many kinds. The GH-25 desalting medium is based on spherical cellulose particles; its 3 kDa exclusion limit allows protein molecules to pass rapidly through the interstitial space while small salt molecules move slowly through the fine pores within the beads, giving desalting and buffer exchange.

The Super Edge empty pre-packed column system allows mini-columns to be self-packed with Cellufine bulk media. It comprises a screw-press packing rod, packing reservoir, empty column kit, quick connectors and related parts.

Covers three types of gel filtration media — high-resolution fine purification (Mixdex), large-molecule separation (Microse Fast Flow and conventional) and rapid desalting and buffer exchange (Micdex) — meeting requirements from molecular weight fractionation and desalting/buffer exchange through to fine purification of large molecules such as viruses and plasmids.

Features: high flow rate and good pressure tolerance; high resolution; very low non-specific adsorption and high recovery; high physicochemical stability, tolerating cleaning with sodium hydroxide and hydrochloric acid at moderate concentrations; a choice of separation ranges.

Features: highly cross-linked agarose with very good flow and pressure characteristics; very low non-specific adsorption and high recovery; stable in ordinary aqueous solutions and tolerant of 8 M urea, 6 M guanidine hydrochloride, 70% ethanol and 1–2 M sodium hydroxide.

Features: lower resolution; very low non-specific adsorption and high recovery; suited to separating biomolecules with widely differing molecular weights (molecular weight determination of polysaccharides, peptides, proteins and similar).

Features: cross-linked dextran microspheres with strong hydrophilicity and very low adsorption, good chemical stability and relatively high resolution; widely used for protein separation, desalting and buffer exchange.

The Mixrose series is based on near-rigid cross-linked agarose microspheres, with near-rigid physical characteristics, a narrower bead distribution and a better-judged mean particle size, combining high binding capacity, low column back pressure, high operating flow rate and high resolution. IEX Microse Fast Flow is among the most widely used media for initial protein capture, with high dynamic binding capacity, high flow rate and retention of capacity after hundreds of CIP cycles. IEX Micdex is a dextran cross-linked ion exchange medium. Functional groups cover strong anion (Q), strong cation (SP), weak anion (DEAE) and weak cation (CM).

Mixed-mode media combine size exclusion with ligand adsorption and other mechanisms, addressing demanding applications such as viral vaccine purification and intermediate or fine antibody purification.

The Mixrose Shell 700, Mixrose Shell 400, Microse Shell V50, Microse Shell V30 and Microse Shell V15 beads have an inert "shell" and an activated "core". Large biomolecules — viruses, virus-like particles, viral vectors, plasmids, exosomes — are excluded from the pores and elute in the void volume, while smaller species such as bovine serum albumin, ovalbumin, host cell protein, nucleases, nucleic acid fragments, endotoxin and pigments enter the pores and are adsorbed by the octylamine mixed-mode ligand coupled to the core, thereby separating and purifying the large biomolecules.

As the preferred medium following antibody affinity purification, Mixrose MMA effectively removes HCP, nucleic acids, viruses, antibody dimers and aggregates, and leached Protein A ligand, improving downstream antibody purification efficiency and reducing cost.

Mixrose MMC HR is a new-generation mixed-mode medium that delivers high resolution at high flow rate, effectively separating aggregates and structural isomers in fine antibody purification.

The PolymerOne® series can produce uniform PS microspheres at scale in any size between 50 nanometres and 1000 micrometres. Surface modification yields PS microspheres bearing different functional groups, including carboxyl, amino and epoxy PS microspheres; microspheres with other cross-linking degrees and functional groups can also be prepared to customer requirement.

Polymer chromatography media are based on a cross-linked organic polymer framework. High-performance polymer reversed-phase media can be prepared on polystyrene/divinylbenzene (PS/DVB) and polymethacrylate (PMMA) matrices. A range of particle sizes (3, 5, 10, 15, 20, 25, 30, 40, 50 μm) is offered, together with optimised pore structures (100, 300, 500 and 800 Å) and a non-porous structure, meeting requirements from analytical to industrial separation.

Uniform polymer reversed-phase media can be supplied in any size from 3 μm to 100 μm, with customisation available. Particle size distribution is measured on a Beckman Coulter Counter, with a coefficient of variation CV<5%.

PolymerOne® polymer reversed-phase media consist of highly cross-linked polystyrene/divinylbenzene or highly cross-linked polymethacrylate, and are stable across the full pH range (pH 1–14). Bead structure and performance remain stable in strongly acidic (1 M HCl) or alkaline (1 M NaOH) solutions and in organic solvents including ethanol, methanol, acetonitrile, isopropanol, acetone, DMSO and tetrahydrofuran.

By adjusting the proportions of the matrix components, polymer reversed-phase media of differing polarity can be supplied: PS/DVB, PSM and PMM, in order of increasing polarity.

Purification processes divide broadly into three steps — crude extraction, intermediate purification and fine purification. PolymerOne® media at 100 μm suit crude extraction, 30 or 40 μm suit intermediate purification and 10 μm suit fine purification, covering the whole separation sequence from crude extract to final polish.

Produced using advanced polymer microsphere preparation and surface modification technology on highly cross-linked uniform polyacrylate (PMMA) or polystyrene/divinylbenzene (PS/DVB) matrices, these media offer high rigidity, acid and alkali resistance, low back pressure, high resolution and high operating flow rate, making them well suited to separation and purification of proteins, oligonucleotides, peptides and oligosaccharides. The PM series forms a hydrophilic coating on the polyacrylate bead surface before introducing ion-exchange groups, eliminating non-specific adsorption and reducing steric hindrance; the M series bonds ion-exchange groups directly to the polyacrylate surface, giving both hydrophobic and ion-exchange modes; the PS/DVB series uses polystyrene/divinylbenzene microspheres with ion-exchange groups bonded directly to the surface.

Built on a highly cross-linked polyacrylate matrix, these media have better mechanical strength than conventional agarose-based ion exchange media. Column volume changes very little at high flow rate and increased pressure, giving high back-pressure tolerance and substantially improved separation efficiency.

PolymerOne® ion exchange chromatography media are based on highly cross-linked polyacrylate microspheres and remain stable across pH 1–14. After 30 days' immersion in 1.0 mol/L NaOH, the dynamic binding capacity for insulin was essentially unchanged.